Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction

Objectives: The objective of the current study was to compare diagnostic methods Oxacillin Disk Diffusion, Cefoxitin Resistance Screening Agar Base, and CHROM MRSA with gold-standard method Polymerase Chain Reaction for detection Methicillin-resistant Staphylococcus aureus.
 Methods: Two hundred pus samples were included in from which strains evaluated. isolates aureus subjected Diffusion test, detect PCR, reference standard. techniques compared their sensitivity, specificity, positive predictive, negative predictive values.
 Results: sensitivity test 100%, followed by at 96.7%, 90%, Base 86.7%. Most specific (99.4%), (98.8%), (97.7%), least (96.5%).
 Conclusion: most sensitive all four methods, next Reaction. However, future multicentric studies are recommended this across prevalent centers methicillin resistance. J Microbiol Infect Dis 2022; 12(3):116-126.